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The cells were continually cultured in comprehensive medium for 48 h, after which the Beckman Coulter MoFlo Astrios Mobile Sorter was utilised to select cells with CXCR4-negative expression. These chosen cells had been cultured in full medium supplemented with 1 μg/mL of puromycin.Ollie123 stated: Surely have not and I'm sorry you will be in the